Figure 4.

Single-Cell RNA-Seq Uncovers a Broad NK Cell-Driven Myeloid Cell Reprogramming from Pro-tumorigenic to Anti-tumorigenic Pathways

Single-cell RNA-seq of 11,651 CD45⁺ cells isolated from pooled tumors from wild-type or NK cell-depleted Ptgs^−/− tumor-bearing mice.

(A) t-SNE plots showing the clustering and distribution. Each point represents a single cell colored according to cluster designation.

(B) Cxcl9 and Cxcl10 gene expression analysis in all cell clusters shown in (A). Data are expressed as normalized counts-per-million (CPM), unpaired Student’s t test.

(C) Enrichment analysis for hallmark IFN-γ response gene set in various monocyte and TAM clusters.

(D) Single-sample GSEA of all hallmark gene sets in the same myeloid populations as in (C).