Supplementary Figure S2: Summary of the processing of WLCs into the various cell types, and representative images for examination of AT-II cell vitality Figure S2: Whole lung epithelial cells from Sftpc-GFP mice were co-cultured with lung fibroblasts in the in vitro colony forming assay in either “MTEC/Plus” or “lung epithelial” culture medium. The growing colonies were collected at 2 wks, immune-stained, and examined for the effect on their differentiation profile. A, B) Representative images showing staining with the ATII and AT-I cell markers, Sftpc and Aquaporin5, in type C colonies. C, D) Representative images showing staining of the A/B colonies with the markers of secretory and basal cells, MUC5AC and Keratin-5. E) Representative image showing staining of the A/B colonies with the markers of ciliated and basal cells, acetylated a-tubulin and Keratin-5. Scale bars A, B = 50 μm, C-E: 100 μm.