Supplementary Figure S3: Effect of mild hyperoxia on lung stem cells proliferation in vitro (related to Figure 4F-I) Figure S3: Whole lung epithelial cells and MTECs were co-cultured with lung fibroblasts in the in vitro colony forming assay in normoxia, hyperoxia of 50%, or hyperoxia followed by normoxia, as described in the results. The colonies and cells were collected, and immune-stained for the proliferation marker PCNA. Representative images showing one colony from each condition, except WLCs hyperoxia where no colonies were detected. Only solitary cells, which were negative for PCNA were detectable. Scale bars = 50 μm.
