Fig. 5.

Immunohistochemistry for Abca1 protein in the yolk sac from LPS treated mice (n = 8/group). A: negative control of technique by primary antibody omission. B and C: Abca1 immunolabeling in the yolk sac from mice at GD15.5 after 4 h of treatment with vehicle (B) and with LPS (C). D, E and F: Abca1 immunolabeling in the yolk sac from mice at GD18.5, after 24 h of treatment with vehicle (D), and after 4 h (E) and 24 h (F) of treatment with LPS. Arrowheads: endodermic epithelium; thin arrows: mesothelium; asterisk: mesodermal blood vessels. G, H, I and J: Semiquantitative evaluation of area and intensity of P-gp immunolabeling. No significant differences were observed. *p < 0,05, Mann Whitney test.