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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: J Physiol. 2020 Sep 27;598(23):5541–5554. doi: 10.1113/JP280276

FIG 4. Application of IFNα in the lungs activates airway nodose C-fibers, visualized by two-photon.

FIG 4.

a. Z stacked images of the same nodose ganglia after application of vehicle and then application of IFNα (1000 U/ml). 1 ml of vehicle or IFNα was delivered into the lungs in 1 min and the response of neurons from the nodose ganglia recorded for a total of 6mins. White bar on the ganglia images represents 100 μm b. Graphs showing calcium transients caused by action potentials arising from lung terminals of 20 representative neurons that were responsive to IFNα (1000 U/ml). X axis denotes the time and Y axis denotes the fluorescence intensity values calculated as change from baseline (𝚫F/F0), n=~300 neurons from 3 mice. c. Graph showing the percentage of capsaicin (CAP) responsive neurons from 3 independent experiments with different concentrations of IFNα (n=~300 neurons from 3 mice, showing mean and SD). A t-test using GraphPad prism software (Bonferroni correction) was used throughout to compare unpaired groups.