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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: J Physiol. 2020 Sep 27;598(23):5541–5554. doi: 10.1113/JP280276

FIG 6. Delivery of IFNb into the mouse airways causes defensive reflexes that require interferon type I receptors.

FIG 6.

a. Representative traces of pulmonary airflow waveforms from mice intratracheally treated with 2ul of saline(blue), IFNβ (2000U, red), and IFNβ + IFN type I receptor blocking antibody (black). The blocking antibody (1mg/kg) was given to the mice i.p. 4 hours before the experiment. Red asterisk denotes a respiratory pause and black shows an augmented breath b. Summary of the total duration of respiratory pauses during a 15-minute interval immediately after administration of 2ul of saline(blue), IFNβ (2000U), and IFNβ + IFN type I receptor blocking antibody. (graph shows mean and SD, n=6 in saline and IFNβ groups and n=5 in IFNb + IFN type I receptor blocking antibody group). IFNβ significantly increased the duration of respiratory pauses when delivered intratracheally in mice (***P<0.0002). IFN type I receptor blocking antibody was able to significantly block the IFNβ effect (**P<0.005). c. Summary of the total number of respiratory pauses during a 15-minute interval immediately after administration of 2ul of saline(blue), IFNβ (2000U), and IFNβ + IFN type I receptor blocking antibody. (graph shows mean and SD, n=6 in saline and IFNβ groups and n=5 in IFNβ + IFN type I receptor blocking antibody group). IFNβ significantly increased the duration of augmented breaths when delivered intratracheally in mice (**P<0.005). IFN type I receptor blocking antibody was able to significantly block the IFNβ effect (*P<0.01). A one-way ANOVA test using GraphPad prism software was used throughout to compare unpaired groups.