Figure 1.
Effect of Neurod1 expression on human vestibular schwannoma (VS) cells in vitro. Primary human VS cultures were treated with empty vector (EV) or vectors of different concentration carrying Neurod1. (A) Western blot of lysates from VS cultures treated with adenovirus expressing ND1 (2×109 or 5×109 pfu/ml) or empty vector (EV, 5×109 pfu/ml) and probed with anti-ND1 antibodies followed by anti-β-actin antibodies. (B,C) Primary human VS cultures labeled with EdU. The percentage of EdU positive, S100-positive nuclei to total S100-positive nuclei were counted by 20× microscopic fields and are expressed here as numbers of EdU positive nuclei per DAPI stained nuclei. Cultures treated with 5×109 EV showed significantly more EdU incorporation (***p<0.05) compared to either 2×109 or 5×109 ND1 containing vectors.