Figure 4.
X-gal staining using Rosa26 LacZ reporter reveals the distribution of beta-galactosidase after Postn-cre mediated recombination. Note that only peripheral SCs in mouse cranial nerves of this E12.5 day old mouse are positive. (A) Lateral view of the head with profound expression in nerves to various skin regions. (B) Medial view showing labeling in cranial ganglia and nerves. (C) Detail of the microdissected ear with X-gal stain in vestibular, geniculate and spiral ganglion. The same Rosa26 expression is expected after Postn-cre mediated recombination of the floxed stop codon in the R26ND1 transgenic animal and will reflect the deletion of the floxed Nf2 gene after Postn-cre mediated recombination. Note absence of X-gal staining in the brain or ear (A,C). V, VII, X, XI, XII, corresponding cranial nerves. Scale bar=1 mm in A,B and 100 μm in C.