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. Author manuscript; available in PMC: 2020 Dec 30.
Published in final edited form as: Cell Rep. 2020 Dec 15;33(11):108498. doi: 10.1016/j.celrep.2020.108498

Figure 1. IL-12- and STAT4-Dependent Induction of STAT5 in NK Cells during MCMV Infection.

Figure 1.

(A) RNA-seq was performed on Ly49H+ NK cells on days 0, 2, 4. 7, 14, and 35 of MCMV infection. Normalized counts of Stat5a and Stat5b are displayed.

(B) RNA-seq on WT versus Il12rb2−/− or Stat4−/− from mixed BMC mice on day 2 PI. Normalized counts of Stat5a and Stat5b are displayed.

(C) ChIP-seq was performed for STAT4 and H3K4me3 on WT and Stat4−/− NK cells cultured in media alone (unstim) or with IL-12 and IL-18. Representative gene tracks of mapped STAT4 ChIP (blue tracks) or H3K4me3 ChIP (black tracks) are displayed.

(D) NK cells were cultured in media (unstim) or with IL-12 + IL-18, and RNA-seq was performed after 3 h of culture. Normalized counts of Stat5a and Stat5b are displayed.

Error bars indicate SEM.