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. 2020 Dec 30;18(12):e3001017. doi: 10.1371/journal.pbio.3001017

Fig 2. Unannotated transcripts in mESCs.

Fig 2

(A) The schematic showing the 5 types of unannotated transcripts. (B) The number of unannotated transcripts detected within different numbers of cells. The unannotated ones captured in at least 3 cells were remained, and we analyzed 110 unannotated transcripts detected in all 53 mESCs. The numerical data are listed in S1 Data. (C) Proportions of these 5 types of unannotated transcripts in mESCs. (D) Examples of unannotated transcripts identified in mESCs. TCONS_00202455 corresponds to a part of the Gene Rabggtb sequence. A complete intron between the second and third exon, 25 bp of intron right next to 5′ of the first exon, and 98 bp of intron between the fourth and fifth exon are retained in the RNA. Gene Mfsd10 has 3 annotated transcripts, and TCONS_00249892 contains the intron between the eighth and ninth exon of the known transcripts. TCONS_00044709 matches partial Gene Top2a sequence and contains a complete intron between the 35th and 36th exon of the known transcript. CJ, combination of known junctions; CS, combination of known splice sites; IR, intron retention; ME, mono-exon; mESC, mouse embryonic stem cell; MIR, mono-exon by intron retention.