Table 2. Comparison of SCAN-seq to current TGS base scRNA-seq methods.

Method	Amplification	Sequencing platform	No. of cells in a library	Cell capture efficiency	No. of UMIs per cell	No. of full-length genes per cell	Experiment cost per cell
SCAN-seq	Smart-seq2	Nanopore	10–48	80%	–	8,000	US$3
R2C2 [27–28]	Smart-seq2	Nanopore	1–96	64%	–	532	US$7
ScISOr-Seq [24]	10x Genomics	NGS+Pacbio	>5,000	40%	260	129	US$0.3
ScNaUmi-seq [25]	10x Genomics	NGS+Nanopore	100–1,000	40%	6047	2,427	US$5
RAGE-seq [26]	10x Genomics	NGS+Nanopore	2,500–6,000	18.7%	–	TCR/BCR mRNA	US$0.5

BCR, B cell receptor; NGS, next generation sequencing; RAGE-seq, Repertoire and Gene Expression by Sequencing; R2C2, Rolling Circle Amplification to Concatemeric Consensus; SCAN-seq, single cell amplification and sequencing of full-length RNAs by Nanopore platform; ScISOr-Seq, single-cell isoform RNA-Seq; ScNaUmi-seq, Single-cell Nanopore sequencing with UMIs; scRNA-seq, single-cell RNA sequencing; TCR, T cell receptor; TGS, third-generation sequencing; UMI, unique molecular identifier.