Fig. 7.

hsa-miR-486-5p indirectly regulates the expression of CYP3A4, PXR, and HNF4α via LINC00844 in HepaRG cells. a The mRNA levels of CYP3A4, PXR, and HNF4α were significantly upregulated in HepaRG cells transfected with hsa-miR-486-5p mimics (gray bars) compared to mimic-NC (white bars). Co-transfection of si-LINC00844 with hsa-miR-486-5p mimics abolished the upregulation of CYP3A4, PXR, and HNF4α caused by hsa-miR-486-5p overexpression (black bars). hsa-miR-486-5p overexpression had no significant effect on CYP2E1 mRNA level in the presence of si-NC. However, the addition of si-LINC00844 significantly reduced CYP2E1 expression, which was consistent with results in Fig. 5a. b LINC00844 knockdown specifically reduced the level of hsa-miR-486-5p but not hsa-miR-495-3p in HepaRG cells. Results shown are mean ± SD from three independent experiments. *p < 0.05