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. Author manuscript; available in PMC: 2020 Dec 30.
Published in final edited form as: Radiat Res. 2020 Dec 1;194(6):625–635. doi: 10.1667/RADE-20-00060.1

FIG. 2.

FIG. 2.

FLASH did not elevate vascular eNOS in the brain. Volumetric analysis of eNOS colocalized with lectin was performed. Panel A: Representative images of eNOS immunoreactivity were converted to 3-D renderings using Imaris at one week postirradiation. Scale bars = 70 μm. Panels B and C: Quantitative analysis of eNOS colocalized within 5 μm of lectin-stained microvasculature at 24 h and one week. CONV increased levels of eNOS-lectin at one week compared to control and FLASH. Data shown are the mean ± SEM (n = per group, two images analyzed per animal). *P < 0.05 (ANOVA with Bonferroni’s multiple comparisons test).