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. Author manuscript; available in PMC: 2020 Dec 30.
Published in final edited form as: Radiat Res. 2020 Dec 1;194(6):625–635. doi: 10.1667/RADE-20-00060.1

FIG. 3.

FIG. 3.

FLASH did not reduce immunoreactivity of CLDN5-lectin and OCLN-lectin as CONV irradiation did, at one week postirradiation. Volumetric analysis of markers CLDN5 and OCLN colocalized to lectin were quantified using Imaris software. Panel A: Representative images of CLDN5 and OCLN taken at the stratum radiatum of the hippocampus. Scale bars = 10 μm. Panels B and C: Analysis of OCLN and CLDN5 data at 24 h and one week within hippocampus and subventricular zone. CONV showed a significant drop-off of tight junction proteins at one week postirradiation compared to controls and FLASH. Data are the mean ± SEM (n = per group, two images analyzed per animal). *P ≤ 0.05, **P ≤ 0.01 and ***P ≤ 0.001 (ANOVA with Bonferroni’s multiple comparisons test).