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. Author manuscript; available in PMC: 2020 Dec 30.
Published in final edited form as: Radiat Res. 2020 Dec 1;194(6):625–635. doi: 10.1667/RADE-20-00060.1

FIG. 6.

FIG. 6.

FLASH provides some protection against CONV irradiation-induced tight junction modification at one month after 10 Gy irradiation. Volumetric analysis of OCLN and CLDN5 colocalization with lectin were measured using Imaris software within the stratum radiatum of the hippocampus and SVZ. Panel A: Measurements of OCLN indicate that both FLASH and CONV decreased levels of immunoreactivity within the hippocampus, but did not significantly change levels within the SVZ compared to controls. Panel B: FLASH does not induce a significant decrease in CLDN5 that CONV dose rates do in the hippocampus. FLASH causes a significant increase of CLDN5 compared to both CONV and control. Data shown are the mean ± SEM (n = 6 per group, two images analyzed per animal). *P ≤ 0.05, **P ≤ 0.01 (ANOVA with Bonferroni’s multiple comparisons test).