figure 1.

striatal mn (a), body weights (b), striatal arginase 2 protein (c), volcano plots representing striatal rnaseq (d i. wt, d ii. yac128) and pathway analysis of differentially expressed (de) genes in pre-manifest wt (e i.) and yac128 (e ii.) male and female mice after 1-week subcutaneous mn exposure. weights collected after second subcutaneous injections of 0 or 50mg/kg mncl2*4h2o and demonstrate a main effect of genotype (p=0.04). data presented in a, b, and c are shown as mean ± sem and **** indicates significance level at p<0.001; n=6–8. data presented in d and e are from a second independent experiment with the same mn exposure and animal age. volcano plots (d) indicate fold change in mrna expression, black dots indicate a single mrna and horizontal grey lines mark q-values of 0.10 and 0.05. n=191 and n=239 indicate the number of significantly altered genes at q-value <0.05 and q-value 0.10, respectively. the number of de genes by mn exposure is greater in wt than yac128 by chi-square, χ²=66.3, df=1, p<0.0001. green dots represent significantly altered genes in both wt and yac128 (n=20). top biological networks enriched after mn exposure in wt (e i.; left) and yac128 (e ii.; right) mice. genes in green (upregulated) or red (downregulated) backgrounds indicate those that were significantly de after mn exposure while genes in grey were not de but function as genetic hubs through which de genes may signal or impact. the intensity of the color indicate the degree of fold change. solid grey lines indicate a direct relationship and dotted lines indicate an indirect relationship. arrows indicate the direction of the relationship. data represents gene changes in n=3 in each genotype and exposure.