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. Author manuscript; available in PMC: 2020 Dec 30.
Published in final edited form as: Biochem J. 2020 Oct 30;477(20):3923–3934. doi: 10.1042/BCJ20200351

Figure 1. Effect of bacterial components on pyroptosis in THP-1 cells.

Figure 1.

THP-1 cells were treated with 50 μM of each chemical in the bacterial chemical library for 1 h. After that, pyroptosis was induced with LPS stimulation at 1 μg/ml for 3 h followed by 10 μg/ml treatment of Nigericin for 1 h. (A) LDH activity in the cell culture supernatant was examined. (B) Cell viability was analyzed with MTT conversion. (C) THP-1 cells were stimulated with or without 1 μg/ml LPS for 3 h and Nigericin at the concentration indicated for an additional hour before harvest. The protein expression of HSP90 and HSP70 was analyzed with Western Blot. In (A) and (B), N = 2–3. In (C), data represent one of four independent experiments, N = 4 (ND, not detectible).