Table 2. Functional identification of SARS-CoV-2 CD4+ T cell epitopes.

¹Screened peptide sequences are named based on the protein they are contained within, followed by the number of the first amino acid residue of the peptide in the context of the full protein to the last amino acid residue. Peptides within the "Spike" peptide library correspond with SARS-CoV Spike due to library availability. ² Average fold over background IFN-γ⁺ CD4⁺T cells stimulated with listed peptides. Background is defined as the frequency of IFN-γ⁺ CD4⁺T cells in a well stimulated with vehicle control. The average and standard deviation is from three independent experiments ³Analogous SARS-CoV-2 peptide name in instances where SARS-CoV peptide library had to be used due to reagent availability. ⁴SARS-CoV-2 peptide sequence in instances where SARS-CoV peptide library was used the analogous SARS-CoV-2 peptide sequence is noted.

Peptide Screened1		Fold Over Background2		SARS-CoV-2 Peptide3	SARS-CoV-2 Sequence4
		Avg.	Std. Dev
SARS CoV-2	Mem127-143	2.7	+/-1.5	Mem127-143	TILTRPLLESELVIGAV
SARS CoV-2	NC127-143	5.0	+/-4.0	NC127-143	KDGIIWVATEGALNTPK
SARS CoV-2	NC8-24	3.6	+/-3.3	NC8-24	NQRNAPRITFGGPSDST
SARS CoV	Spike865-882	4.5	+/-3.9	Spike883-900	TSGWTFGAGAALQIPFAM
SARS CoV	Spike954-971	3.2	+/-2.0	Spike972-989	AISSVLNDILSRLDKVEA
SARS CoV	Spike993-1010	4.8	+/-2.9	Spike1011-1028	QLIRAAEIRASANLAATK