. 2020 Dec 8;9:e57063. doi: 10.7554/eLife.57063

Table 1. Gating strategy for the identification of effector/memory CD4⁺T-cell subsets.

Gates 1 and 2	Gate 3	Gate 4	Gate 5	Gate 6	Gate 7	Gate 8	Subset
Live single CD3⁺ CD14⁻ CD19⁻ lymphocytes	CD4⁺	Exclude CCR7⁺ CD45RA⁺	CD25^high CD127^low				Treg
			CD25^low CD127⁺	CXCR5⁺			Tfh
				CCR10⁺			Th22
				CXCR5⁻ CCR10⁻	CXCR3⁺ CCR6⁻	CCR4⁻	Th1
					CXCR3⁻ CCR6⁺	CCR4⁺	Th17
					CXCR3⁺ CCR6⁺	CCR4⁻	Th1-17
					CXCR3⁻ CCR6⁻	CCR4⁺ CRTh2⁻	Th2
					CXCR3⁻ CCR6⁻	CCR4⁺ CRTh2⁺	Th2a

Table 1. Gating strategy for the identification of effector/memory CD4+ T-cell subsets.