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. 2020 Dec 22;9:e58037. doi: 10.7554/eLife.58037

Figure 7. IL-1β-Induced HSV Reactivation is linked to heightened neuronal excitability and dlk activation.

Figure 7.

(A) Quantification of Us11-GFP expressing neurons following addition of IL-1β to latently infected cultures of mature SCG neurons. (B) Numbers of Us11-GFP-positive neurons following addition of forskolin or IL-1β to mature SCG neurons, and in the presence of an IL-1R-blocking antibody (2 μg/mL). (C) Quantification of IL-1β induced reactivation in the presence of the voltage-gated sodium channel blocker TTX (1 μM), the HCN-channel blocker ZD 7288 (10 μM) and the DLK inhibitor GNE-3511 (4 μM). (D) Latently infected SCG neurons were transduced with an shRNA control lentivirus or lentiviruses expressing shRNA against DLK. Three days later IL-1β was added to the cultures and the numbers of GFP-positive neurons quantified at 3 days later. Individual experimental replicates, means and SEMs are represented. Statistical comparisons were made using two-tailed unpaired t-test (A) or a one-way ANOVA with a Tukey’s multiple comparison (B–D). *p<0.05, **p<0.01.

Figure 7—source data 1. Quantification of GFP-positive neurons for Figure 7.