Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus, M/F) | CD1 | Charles River | Crl:CD1(ICR) | |
| Strain, strain background (Human herpesvirus 1) | HSV Us11-GFP | I gift from Ian Mohr, NYU. PMID:12915535 | ||
| Strain, strain background (Human herpesvirus 1) | HSV-1 17syn+ | A gift from Roger Everett, MRC Virology Unit Glasgow | ||
| Cell line (Homo sapiens) | 293LTV | Cell Biolabs | Cat # LTV-100 RRID:CVCL_JZ09 |
|
| Cell line (Cercopithecus aethiops) | Vero | ATCC | Cat # CCCL-81 RRID:CVCL_0059 |
|
| Recombinant DNA reagent | pCMV-VSV-G | A gift from Bob Weinberg/Addgene PMID:12649500 | Cat # 8454 RRID:Addgene_8454 |
|
| Recombinant DNA reagent | psPax2 | A gift from Didier Trono/Addgene | Cat # 12260 RRID:Addgene_12260 |
|
| Antibody | Anti-phospho-Akt (S473) (Rabbit monoclonal) | Cell Signalling Technologies | Cat # 4060 RRID:AB_2315049 |
WB (1:500) |
| Antibody | Anti-Akt (pan) (Rabbit monoclonal) | Cell Signalling Technologies | Cat # C67E7 RRID:AB_915783 |
WB (1:1000) |
| Antibody | Anti-phopsho-c-Jun (Rabbit monoclonal) | Cell Signalling Technologies | Cat # 3270 RRID:AB_2129575 |
WB (1:500) |
| Antibody | Anti-DLK/MAP3K12 (Rabbit polyclonal) | Thermo Fisher | PA5-32173 RRID:AB_2549646 |
WB (1:500) |
| Antibody | Anti-a-tubulin (Mouse monoclonal) | Millipore sigma | Cat # T9026 RRID:AB_477593 |
WB (1:2500) |
| Antibody | Anti-Rabbit IgG Antibody (H+L), Peroxidase (Goat polyclonal) | Vector Labs | Cat # PI-1000 RRID:AB_2336198 |
WB (1:10000) |
| Antibody | Anti-mouse IgG Antibody (H+L), Peroxidase (Horse polyclonal) | Vector Labs | Cat # PI-2000 RRID:AB_2336177 |
WB (1:10000) |
| Antibody | Anti- H3K9me3S10P (Rabbit polyclonal) | Abcam | Cat # Ab5819 RRID:AB_305135 |
IF (1:250) |
| Antibody | Anti-Beta-III Tubulin (Chicken polyclonal) | Millipore Sigma | Cat # AB9354 RRID:AB_570918 |
IF (1:1000) |
| Antibody | Anti-γH2A.X (Mouse monoclonal) | Cell Signalling Technologies | Cat # 80312 RRID:AB_2799949 |
IF (1:100) |
| Antibody | Anti-c-Fos (Rabbit polyclonal) | Novus | Cat # NB110-75039 RRID:AB_1048550 |
IF (1:125) |
| Antibody | F(ab’)2 Anti-Mouse IgG (H+L) Alexa Fluor 647, (Goat polyclonal) | Thermo Fisher | Cat # A21237 RRID:AB_2535806 |
IF (1:1000) |
| Antibody | F(ab’)2 Anti-Rabbit IgG (H+L) Alexa Fluor 555 (Goat polyclonal) | Thermo Fisher | Cat # A21425 RRID:AB_2535846 |
IF (1:1000) |
| Antibody | Anti-Chicken IgY (H+L) Alexa Fluor 647 (Goat pAb) | Abcam | Cat # Ab150175 RRID:AB_2732800 |
IF (1:1000) |
| Antibody | Anti-Chicken IgY (H+L) Alexa Fluor 488 (Goat polyclonal) | Abcam | Cat # Ab150173 RRID:AB_2827653 |
IF (1:1000) |
| Antibody | F(ab’)2 Anti-Rabbit IgG (H+L) Alexa Fluor 488 (Goat polyclonal) | Thermo Fisher | Cat # A-11070 RRID:AB_2534114 |
IF (1:1000) |
| Antibody | Anti-Mouse IL-1R (Goat polyclonal) | Leinco Technologies | Cat # I-736 RRID:AB_2830857 |
Blocking (2 ug/mL) |
| Sequence-based reagent | mGAP F | PMID:19515781 | PCR primers | CATGGCCTTCCGTGTGTTCCTA |
| Sequence-based reagent | mGAP R | PMID:19515781 | PCR primers | GCGGCACGTCAGATCCA |
| Sequence-based reagent | ICP27 F | PMID:21285374 | PCR primers | GCATCCTTCGTGTTTGTCATTCTG |
| Sequence-based reagent | ICP27 R | PMID:21285374 | PCR primers | GCATCTTCTCTCCGACCCCG |
| Sequence-based reagent | ICP8 F | PMID:23322639 | PCR primers | GGAGGTGCACCGCATACC |
| Sequence-based reagent | ICP8 R | PMID:23322639 | PCR primers | GGCTTAAATCCGGCATGAC |
| Sequence-based reagent | ICP4 F | This paper | PCR primers | TGCTGCTGCTGTCCACGC |
| Sequence-based reagent | ICP4 R | This paper | PCR primers | CGGTGTTGACCACGATGAGCC |
| Sequence-based reagent | UL30 F | PMID:22383875 | PCR primers | CGCGCTTGGCGGGTATTAACAT |
| Sequence-based reagent | UL30 R | PMID:22383875 | PCR primers | TGGGTGTCCGGCAGAATAAAGC |
| Sequence-based reagent | UL48 F | This paper | PCR primers | TGCTCGCGAATGTGGTTTAG |
| Sequence-based reagent | UL48 R | This paper | PCR primers | CTGTTCCAGCCCTTGATGTT |
| Sequence-based reagent | gC F | This paper | PCR primers | CAGTTTGTCTGGTTCGAGGAC |
| Sequence-based reagent | gC R | This paper | PCR primers | ACGGTAGAGACTGTGGTGAA |
| Sequence-based reagent | shRNA: DLK-1 | Broad Institute: Genetic Perturbation Platform/Millipore Sigma | TRCN0000022573 | |
| Sequence-based reagent | shRNA: DLK-2 | Broad Institute: Genetic Perturbation Platform/Millipore Sigma | TRCN0000022572 | |
| Sequence-based reagent | shRNA: non-targeting control | PMID:16873256 | ||
| Commercial assay or kit | Quick-RNA Miniprep | Zymo Research | R1054 | |
| Commercial assay or kit | SuperScript IV First-Strand Synthesis System | ThermoFisher | 18091050 | |
| Commercial assay or kit | SYBR Green PCR Master Mix | ThermoFisher | 4309155 |
|
| Chemical compound, drug | Acycloguanosine | Millipore Sigma | A4669 | 10 µM, 50 µM |
| Chemical compound, drug | FUDR | Millipore Sigma | F-0503 | 20 µM |
| Chemical compound, drug | Uridine | Millipore Sigma | U-3003 | 20 µM |
| Chemical compound, drug | SP600125 | Millipore Sigma | S5567 | 20 µM |
| Chemical compound, drug | GNE-3511 | Millipore Sigma | 533168 | 4 µM |
| Chemical compound, drug | GSK-J4 | Millipore Sigma | SML0701 | 2 µM |
| Chemical compound, drug | L-Glutamic Acid | Millipore Sigma | G5638 | 3.7 µg/mL |
| Chemical compound, drug | Forskolin | Tocris | 1099 | 60 µM |
| Chemical compound, drug | LY 294002 | Tocris | 1130 | 20 µM |
| Chemical compound, drug | 666–15 | Tocris | 5661 | 2 µM |
| Chemical compound, drug | SQ 22,536 | Tocris | 1435 | 50 µM |
| Chemical compound, drug | KT 5720 | Tocris | 1288 | 3 µM |
| Chemical compound, drug | TEA | Tocris | 3068 | 10 mM |
| Chemical compound, drug | CsCl | Tocris | 4739 | 3 mM |
| Chemical compound, drug | OG-L002 | Tocris | 6244 | 30 µM |
| Chemical compound, drug | S2101 | Tocris | 5714 | 20 µM |
| Chemical compound, drug | Tetrodotoxin | Tocris | 1069 | 1 µM |
| Chemical compound, drug | ESI-09 | Tocris | 4773 | 10 µM |
| Chemical compound, drug | ZD 7288 | Cayman | 15228 | 20 µM |
| Chemical compound, drug | 8-bromo-cyclic AMP | Cayman | 14431 | 125 µM |
| Chemical compound, drug | NGF 2.5S | Alomone Labs | N-100 | 50 ng/mL |
| Chemical compound, drug | Primocin | Invivogen | ant-pm-1 | 100 µg/mL |
| Chemical compound, drug | Aphidicolin | AG Scientific | A-1026 | 3.3 µg/mL |
| Chemical compound, drug | IL-1β | Shenendoah Bio. | 100–167 | 30 ng/mL |
| Chemical compound, drug | WAY-150138 | Pfizer, gift from Lynn Enquist and Jay Brown. | NA | 10 µg/mL |
| Chemical compound, drug | Fura-2 AM | Thermo Fisher | F1221 | 5 µM |
| Other | Hoescht Stain | Thermo | 62249 | 2 µM |