Figure 8. Effects of short-term IPR-179 treatment on epileptogenesis in the intrahippocampal KA mouse model.

Experimental schedule with key milestones (A). Two-way RM ANOVA revealed the effects of IPR-179 treatment on epileptiform spike epoch counts (F_1,14 = 6.974, P = 0.019) (B) and total number of seizures (F_1,14 = 9.281, P = 0.009) (C). The average seizure duration during the week of IPR-179 treatment was also reduced (F_1,14 = 6.413, P = 0.024) (D). Epileptiform activity as a function of the circadian hour (E, left) and the circadian phase (E, right) was averaged for all EEG-monitoring days. Two-way RM ANOVA revealed differences between groups (F_1,14 = 9.117, P = 0.009 and F_1,14 = 8.862, P = 0.010, respectively). The effects of short-term IPR-179 treatment on memory deficits associated with epileptogenesis in mice were evaluated with the novel object recognition test (F) and novel object location test (G). Data are represented as mean + SEMs; dots in histograms represent individual samples; box-and-whisker plots display median with 5–95 percentiles. The log₁₀ scale is used for B, C, and D. Vehicle, n = 7; IPR-179, n = 9. Two-way RM ANOVA on ranks was applied for panels B, C, and D due to non-Gaussian distribution of values. ⁺P < 0.1; *P < 0.05; **P < 0.01; ***P < 0.001, Holm-Šidák post hoc test. A paired t test was applied to compare exploration times within the same treatment group. A nonpaired t test was used to compare the discrimination ratios between treatment groups.