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. 2021 Jan 4;131(1):e140177. doi: 10.1172/JCI140177

Figure 3. Chemokine receptor expression in young and old C57BL/6 mouse BM collected under hypoxia and processed under ambient air or hypoxic conditions.

Figure 3

Young and old C57BL/6 donor BM cells were collected and processed as in Figure 1A. CXCR4 expression on LT-HSCs (A), ST-HSCs (B), MPPs (C), CMPs (D), GMPs (E), and MEPs (F). Data represent the mean ± SEM for 6 C57BL/6 mice per group. (G) Experimental schema to determine the homing capacity of donor cells. (H) Percentage of CD45.1–CD45.2+ cells in BM determined 18 hours after injection. (I) Number of CD45.1–CD45.2+ (LSK CD150+) HSCs in BM determined 18 hours after injection. (H and I) Data represent the mean ± SEM of 10 femurs per group. (J) Myeloid (CD11b+)/lymphoid (CD3+ and B220+) ratio in BM 6 months after primary transplantation. Data represent the mean ± SEM for 4–6 F1 recipient mice. (K) CCR5 expression on LT-HSCs. Data represent the mean ± SEM for 6 C57BL/6 mice per group. (L) CCR2 expression on LT-HSCs. Data represent the mean ± SEM for 3 C57BL/6 mice per group. *P < 0.05, **P < 0.01, and ***P < 0.001, by 1-way ANOVA with post hoc Tukey’s multiple-comparison test.