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. 2021 Jan 4;131(1):e140966. doi: 10.1172/JCI140966

Figure 2. C3aR/VCAM1 axis promotes peripheral lymphocyte infiltration during aging.

Figure 2

(A) Representative CD45 and CD11b flow cytometric plots and gating strategy of dissociated mouse brain at 2 months, 12 months, and 20 months. LY, lymphocyte; MN, monocyte; MG, microglia; DN, double-negative. (B) Flow cytometric analysis and quantification of percentage of infiltrating lymphocytes (2 months n = 12, 12 months n = 7, 20 months n = 12) showed age-related increase. (C and D) Flow cytometry analysis and quantification of brain lymphocytes in 12–14-month-old WT (n = 9) and C3ar1–/– (n = 10) mice showed reduction in aged C3ar1–/– mice. All data represent the mean ± SEM. Significance was calculated using 1-way ANOVA with Tukey’s post hoc test (**P < 0.01, ***P < 0.001).