Skip to main content
. 2021 Jan 4;131(1):e139807. doi: 10.1172/JCI139807

Figure 1. Differentiation of hiPSCs to SCPs.

Figure 1

(A) Pluripotency of isogenic NF1+/+, NF1+/–, and NF1–/– hiPSCs was confirmed by expression of NANOG, Oct3/4, SOX2, and TRA1-60. (B) Differentiation of isogenic hiPSCs into SCPs was confirmed by negative staining for Oct3/4 on day 6 and positive staining for p75, SOX10, and HOXb7 on day 10. Yellow arrows depict the colocalization of SOX10 and HOXb7 in hiPSC-SCPs. (C) Differentiation of isogenic hiPSCs into SCPs was confirmed by positive staining for GAP43 and nestin on day 10 and for SOX10 and AP2a on day 20, respectively. The morphology was distinct from that of original hiPSCs 10 days after differentiation. BF, bright-field. Scale bar: 50 μm.