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. 2021 Jan 4;131(1):e130740. doi: 10.1172/JCI130740

Figure 3. MiR-146a is upregulated in the epidermis of psoriatic lesions; this miRNA promotes production of the proinflammatory cytokines IL-6 and IL-8.

Figure 3

(A) MiR-146a in the epidermis of normal and psoriatic skin was detected by RNA in situ hybridization assay. Scale bars: 100 μm. (B) The miR-146a level in HaCaT cells treated with IL-17A was quantified by real-time PCR. (C and D) A miR-146a–overexpressing vector (pmiR-146a) and a scrambled control hairpin in pCDH-CMV-MCS-EF1-copGFP (pmiR) from SBI (System Biosciences) were used to generate stable miR-146a–overexpressing and control cell clones, respectively. The secretion of cytokines (IL-6 and IL-8) by HaCaT cells stably transfected with pmiR or pmiR-146a vectors was measured 2 days after stimulation with 25 or 100 ng/mL IL-17A. Three independent biological replicates were performed for the real-time PCR analysis. All results are presented as mean ± SD. For statistical analysis, unpaired Student’s t test (B) or 2-way ANOVA with Tukey’s multiple-comparison test (C and D) was performed. **P < 0.01, ***P < 0.001.