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. 2021 Jan 4;131(1):e139481. doi: 10.1172/JCI139481

Figure 2. Epithelial NF-κB activation in saccular-stage lungs results in lifelong abnormalities in lung structure and function.

Figure 2

Dams were treated with Dox from P3 to P5 (saccular stage), after which lungs from IKTA and littermate control mice were harvested at 2, 6, or 24 months. (A) Representative image showing gross appearance of a 24-month IKTA lung. Arrows denote areas of emphysema. (B and C) Representative images showing H&E-stained (B) and Hart’s elastin–stained (C) lung sections from 24-month control and IKTA lungs. Arrows denote intact elastic fibers. Arrowhead denotes fragments of elastic fibers. (D and E) Quantification of mean linear intercept (MLI) (D) and alveolar attachments (E) in control and IKTA lungs at 2, 6, and 24 months. Data are expressed as mean ± SEM, n = 3–4 lungs per group, 6 imaged lung fields per lung *P < 0.05 by 2-tailed Student’s t test comparing IKTA to littermate controls at each age. (F–H) Lung function, including total respiratory system elastance (F), tissue elastance (G), and dynamic compliance (H), was measured at 2, 6, and 24 months in control and IKTA mice. Data are expressed as mean ± SEM, n = 4–6 mice per group, *P < 0.05 by 2-tailed Student’s t test comparing IKTA to littermate controls at each age, #P < 0.05 by 1-way ANOVA and post hoc linear trend test for each group (control and IKTA). Scale bar: 100 μm in B, 50 μm in C.