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. 2021 Jan 4;131(1):e139481. doi: 10.1172/JCI139481

Figure 3. Transgene activation results in neutrophilic inflammation during both the saccular and alveolar stages of lung development.

Figure 3

Dams were treated with Dox from P3 to P5 (saccular stage) or P10 to P12 (alveolar stage), after which lungs from IKTA and littermate control mice were harvested at P5 or P12. (A) Expression of constitutively active IKK-β (cIkk-β) transgene and Gapdh was evaluated by PCR followed by agarose gel electrophoresis. (B and C) Quantification of immune cells in P5 (B) and P12 (C) control and IKTA lungs by flow cytometry. Data are expressed as mean ± SEM, n = 3–4 per group. *P < 0.05 by 2-tailed Student’s t test. (D and E) mRNA expression of select inflammatory cytokines in P5 (D) and P12 (E) control and IKTA lungs. Data are expressed as mean ± SEM, n = 4–6 per group.