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. 2021 Jan 4;131(1):e139481. doi: 10.1172/JCI139481

Figure 4. Neutrophilic inflammation disrupts saccular-stage (but not alveolar-stage) elastic fiber assembly and results in a BPD-like phenotype.

Figure 4

Dams were treated with Dox from P3 to P5 (saccular stage) or P10 to P12 (alveolar stage), after which lungs from IKTA and littermate control mice were harvested at P5 or P12. (A, B, E, and F) Representative H&E-stained lung sections (A and E) and morphometric measurements (B and F) demonstrated dilated terminal saccules in P5 IKTA lungs, whereas P12 IKTA lungs appeared similar to controls. Data are expressed as mean ± SEM, n = 4–5 lungs per group, 5–6 imaged lung fields per lung, *P < 0.05 by 2-tailed Student’s t test. (C and G) Representative photomicrographs of Hart’s elastin–stained sections of P5 (C) and P12 (G) control and IKTA lungs demonstrating disorganized and fragmented elastic fibers in P5 IKTA lungs. Arrows denote normal elastic fibers in controls. Arrowhead denotes fragmented elastic fibers in IKTA lungs. (D and H) mRNA expression of elastic fiber assembly components Eln and Fbln5 expression in P5 (D) and P12 (H) IKTA lungs. Data are expressed as mean ± SEM, n = 4–5 per group, *P < 0.05 by 2-tailed Student’s t test. Scale bar: 100 μm in A and E, 50 μm in C and G.