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. 2021 Jan 4;131(1):e139481. doi: 10.1172/JCI139481

Figure 5. Neutrophil depletion rescues lung development in saccular-stage lungs with NF-κB activation.

Figure 5

Control and IKTA pups were administered anti-ly6G antibodies or control IgG by i.p. injection on P2 prior to placing dams on Dox from P3 to P5 for transgene activation. (A and B) Representative flow plots (A) and quantification of CD11b+ Ly6G+ neutrophils (B) showing reduction in neutrophils with anti-ly6G antibody treatment. Data are expressed as mean ± SEM, n = 3–4 lungs per group, *P < 0.05 by 1-way ANOVA and post hoc Tukey test. (C–E) Representative H&E-stained lung sections (C), morphometric evaluation of average distal airspace area (D), and average distal airspace perimeter (E) in P5 lungs. Data are expressed as mean ± SEM, n = 4 lungs per group, 4–6 imaged fields per lung (D and E). *P < 0.05 by 1-way ANOVA and post hoc Tukey test. Scale bar: 100 μm in C.