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. 2021 Jan 4;131(1):e134529. doi: 10.1172/JCI134529

Figure 7. TLR3 ablation decreases ISG expression and increases vulnerability to viruses in MEFs.

Figure 7

(A) Gene expression profile of all differentially expressed ISGs in Tlr3–/– MEFs relative to mean levels in WT mice, as assessed by RNA-Seq. The heatmap shows the log fold-changes of ISG gene expression, with red indicating upregulation and green downregulation. (B) ISG expression was assessed in unstimulated WT and Tlr3–/– MEFs, by RT-qPCR with normalization against RPL19. WT and Tlr3–/– MEFs were infected with VSV-WT (C) and VSV-M51R (D) for 24 hours. Viral RNA levels were then quantified by RT-qPCR, with normalization against RPL19. The error bars indicate SDs of technical triplicates.