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. 2021 Jan 4;131(1):e128114. doi: 10.1172/JCI128114

Figure 12. Reexpression of LDLR with NPVY motif rescues hypomyelination in Ldlr–/– mice.

Figure 12

(A) Representative fluorescence images of the transfection of GFP (green) reporter lentiviruses into mature oligodendrocytes (GST-pi+, red) with re-expression of LDLR (dark blue, indicated by white arrows) in the CC of Ldlr–/– mice. (B) Images showing the proliferation of native OPCs (n = 4 in each group). (C) Quantification of the results in B. (D) Images showing the mature oligodendrocytes (CC1+ or GST-pi+ cells) in the CC (n = 4 in each group). (E) Quantification of the results in D. (F) Representative fiber tractographies of mice injected with 3 types of viruses. Eigenvector components were mapped to the following color schemes: red = left–right, blue = cranial–caudal, and green = dorsal–ventral directions (n = 3 in each group). (G and H) Quantification of the results in F. (I) MBP and MAG staining of the CC in Ldlr–/– mice receiving lentiviruses (n = 4 in each group). The CC boundary is indicated by the white dashed line. (J and K) Quantification of the results in I. Data presented as mean ± SD. *P < 0.05, **P < 0.01 vs. LV-control by 1-way ANOVA with Tukey’s post hoc test. Scale bars: 20 μm.