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. 2021 Jan 4;131(1):e140424. doi: 10.1172/JCI140424

Figure 1. Generation of mice with specific deletion of both REV-ERBα and –β in non-SCN hypothalamic nuclei.

Figure 1

(A) Diurnal expression of HA-REV-ERBα in ARC, VMH, and DMH from HA-REV-ERBα mice housed in regular 12-hour light/12-hour dark cycles. Percentage of HA-positive cells was determined over total number of cells detected by DAPI staining (n = 6–10, mean ± SEM, double-plotted). Representative pictures of HA-REV-ERBα staining in the ARC and VMH at the peak (ZT1) and trough (ZT13) are presented. Scale bars: 100 μm. 3V, third ventricle. (B and C). Reverbα, Reverbβ, and canonical REV-ERB target gene expression in the ARC (B) and VMH/DMH (C) from Reverbαfl/fl-Reverbβfl/fl-Nkx2.1-Cre+ mice (named HDKO mice) compared with their control littermates (Reverbαfl/fl-Reverbβfl/fl-Nkx2.1-Cre–) at ZT4 on NCD (n = 4–5, mean ± SEM). Results were compared by unpaired t test. **P < 0.01, ***P < 0.001.