Supernatant concentrations of palmitoylcarnitine 24 hours after incubation with the intestinal cell lines (A) Caco2, (B) LS174t, and (C) T84. (D) Basolateral versus apical uptake of palmitoylcarnitine after 24-hour incubation with T84 cell monolayers grown on Transwell plates to a high transepithelial resistance (TER). (E) Palmitoylcarnitine uptake in intestinal cell lines 24 hours after incubation with the mitochondrial inhibitor piericidin A or (F) the peroxisomal inhibitor thioridazine. n = 3, mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001. P values represent a 2-tailed Student’s t test and paired-sample t test.