Skip to main content
. 2021 Jan 4;131(1):e138986. doi: 10.1172/JCI138986

Figure 5. EC-SEVs contain high levels of ANGPTL2 protein.

Figure 5

(A) The experimental procedure for mass spectra analysis. The SEVs were collected from the culture supernatant of WT ECs, Vps33b-knockdown ECs, and normal BM fluid. The proteins were extracted from the SEVs for mass spectrometry. (B) Mass spectra results. The overlap in the proteins enriched in WT EC-SEVs versus BM-SEVs and WT EC-SEVs versus Vps33b-knockdown (KD) EC-SEVs is shown. (C) Western blot analysis of ANGPTL2 (A2), β-actin, and CD63 protein levels in BM ECs and EC-SEVs. (D) qRT-PCR detection of Angptl2 mRNA in the indicated cells (n = 3; the data represent the means ± SD). β-Actin was used as an internal control. Experiments were conducted 2–3 times for validation.