Figure 9. ANGPTL2-SEVs can target human LSCs.

(A) The experimental procedure for studying the effect of ANGPTL2-SEVs on human AML cells. 293T cells were transduced with Ctrl vectors or ANGPTL2 vectors. Ctrl-SEVs or ANGPTL2-SEVs were collected from the supernatant of the transduced 293T cells. Human AML cells (1 × 10⁶) were cocultured with 10 μg Ctrl-SEVs or ANGPTL2-SEVs, and then colony assay and transplantation into NOD/SCID mice were performed. (B) Images of CD34⁺ human AML cells captured after coculture with CFSE-labeled SEVs. Scale bar: 5 μm. (C) Flow cytometry (left) and histogram (right) analysis of the CFSE signals of CD34⁺ human AML cells (n = 3; the data represent the means ± SD; ***P < 0.001, Student’s t test). Experiments were conducted 3 times for validation.