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. 2021 Jan 4;131(1):e134565. doi: 10.1172/JCI134565

Figure 4. USP7 preferentially interacts with soluble polyQ-expanded AR and does not colocalize with nuclear inclusions.

Figure 4

IP of AR112Q-expressing PC12 cell protein lysates with anti-USP7 antibody, resolved by SDS-PAGE (A) or SDS-agarose (B). (C) Quantification of slow- and fast-migrating species from input lanes of B. (D) Immunofluorescence images of PC12 cells expressing AR112Q. Arrow points to a nuclear inclusion. Scale bar: 10 μm. (E) AR immunofluorescence images of cells examined by PLA in Figure 2C. Approximately 10% (9.5 ± 1.1) of AR112Q-expressing cells contained nuclear inclusions. Scale bar: 10 μm.