Figure 7. Zeb1^–/– EpCAM⁺ HSPCs display enhanced cell survival and diminished mitochondrial metabolism, RNA biogenesis, and differentiation transcriptional signatures.

(A) Volcano plot showing the relationship between magnitude of gene expression change (log₂ of fold-change; x axis) and statistical significance of this change (–log₁₀ of adjusted P value; y axis) in a comparison of Zeb1^–/– EpCAM⁺ to Zeb1^–/– EpCAM^– LSK cells. Colored points represent DEGs (cutoff FDR < 0.05) that are either overexpressed (red) or underexpressed (green) in Zeb1^–/– EpCAM⁺ compared with Zeb1^–/– EpCAM^–. (B) GSEA plots of regulation of apoptosis, stabilization of P53, TP53 targets phosphorylated, and HSC differentiation. Heatmaps of the DEGs within EpCAM⁺ and EpCAM^– LSK after Zeb1 deletion related to antiapoptosis (C) and proapoptosis (D). (E) Representative histogram of BCL-XL levels in EpCAM fractions within Zeb1^–/– LSK. (F) Canonical pathways that were mostly enriched in Zeb1^–/– EpCAM⁺ LSK cells derived from the IPA, BioCarta, KEGG, PID, and Reactome pathway databases. Data are shown as –log₁₀ (P value), and the dashed black line indicates P value of 0.05. Analysis was performed using the GSEA software and IPA.