Fig. 12.

A schematic illustration of the microfluidic perfusion system. (A) An expanded view showing the configuration of the microdevice with a microfluidic perfusion chamber and an integrated temperature-controlled stage. (B) A diagram showing the connections between the microdevice and three syringes and the microchannels. The arrows indicate the direction of fluid flow. The HP Agilent 34970A data acquisition system is used for monitoring and recording the temperature with the thermocouple. δ1–δ6, thickness; S1–S3, syringes; A1–A3, representative cross sections; P1– P3, typical cell locations. Note: drawing not to scale [124].