Fig. 5.

Activation of Nrf2 induce osteocyte-specific gene expression. (A and B) mRNA expression levels of osteoblastic and osteocytic genes in primary osteoblasts (A) (n = 9) and primary osteocytes (B) (n = 9) wild type and knockout for NRF2. (C) mRNA expression in primary osteocytes infected with NRF2 expression vectors (n = 6). mRNA expression levels were measured by RT-qPCR and normalized to Tbp expression. Results were plotted as expression relative to cells infected with GFP vector (mean ± SEM of six to eight independent experiments). *p < 0.05, **p < 0.01, and ***p < 0.001 using Student's t-test.