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. 2020 Dec 17;11:606575. doi: 10.3389/fphys.2020.606575

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Copyright © 2020 Song, Wang, Liu, Sun and Ban.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunocytochemical localization OBP6, OBP7, and OBP8 in sensilla placoidea on the 3rd and 6th segments of antennae in spotted alfalfa aphid. The preimmune serum was used as control. The antiserum against OBP6 of T. trifolii specifically and strongly labeled sensilla placoidea on the 6th segment and 3rd segment. The lymph of placoid sensilla on the 3rd segment was also labeled specifically by OBP8, but weaker than OBP6. The antiserum against OBP7 showed relatively weak, but significant staining specifically in the placoid sensilla of the 3rd antennal segment. Dilution of primary antibody was 1:1,000, and that of the secondary antibody was anti-rabbit IgG conjugated with 10 nm colloidal gold granules at a dilution of 1:20; the same below.