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. 2020 Dec 17;11:606575. doi: 10.3389/fphys.2020.606575

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Copyright © 2020 Song, Wang, Liu, Sun and Ban.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunocytochemical localization of OBP6 (B), OBP7 (C), and OBP8 (D) in sensilla stellate on the antennae of spotted alfalfa aphid. The preimmune serum was used as control (A). OBP6, OBP7, and OBP8 are densely labeled in the sensillum lymph of stellate sensilla and appear to be colocalized in the same sensillum while preimmune serum shows no labeling at all. Dilution of the primary antibody was 1:1,000, and that of the secondary antibody was anti-rabbit IgG conjugated with 10 nm colloidal gold granules at a dilution of 1:20.