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Over-expression of pORF5 in transfected HeLa cells. (A,B) The protein and mRNA expression levels of pORF5 were tested by western blot and qRT-PCR, respectively. (C) The expression of pORF5 detected by IFA. DNA (blue) was stained with Hoechst 33258, GFP (green) was expressed by transfected plasmid. pORF5 (red) was stained with Cy3 (200×).
