Figure 5.

Villus and crypt cell sub-type counts are affected by the timing of first feeding. (A) Villus cell sub-type counts by IF between Hatch and d10 in DF chicks (left) and EF chicks (right). Cells were either FABP⁺/PCNA^– (purple bars), or FABP⁺/PCNA⁺ (lined bars). (B) Crypt cell sub-type counts by IF between Hatch and d10 in DF chicks (left) and EF chicks (right). Cells were either Sox9⁺/PCNA⁻ (teal bars), Sox9⁺/PCNA⁺ (lined bars), Sox9⁻/PCNA⁺ (yellow bars), or Sox9⁻/PCNA⁻ (gray bars). Values are means + SEM, asterisks mark significant differences between DF and EF at each age by t-test, p < 0.05.