Figure 6.

Villus and crypt goblet cell counts between Hatch and d10 are affected by the timing of first feeding. Goblet cells were quantified by counting Alcian blue-periodic acid Schiff (AB-PAS) mucin staining in villi (A) and crypts (B) between Hatch and d10 in DF and EF chicks. Values are means + SEM, and asterisks mark significant differences between DF and EF chicks for each age by t-test, p < 0.05. (C) Representative images of AB-PAS staining at d10 in DF and EF chicks. Scale bars indicate 50 μm.