Table 1.
Villus and crypt cell sub-type ratios between Hatch and d10 in delayed feeding (DF) and early feeding (EF) chicks.
| Age (days) | EF/DF | Villus cell ratios (%) | Crypts cell ratios (%) | ||||
|---|---|---|---|---|---|---|---|
| FABP+/PCNA+ | FABP+/PCNA− | Sox9+/PCNA− | Sox9+/PCNA+ | Sox9−/PCNA+ | Sox9−/PCNA− | ||
| Hatch | - | 13.4 ± 1.1 | 86.6 ± 1.1 | 17.8 ± 2 | 49.7 ± 2.6 | 19 ± 1.8 | 13.5 ± 2.4 |
| D1 | DF | 9.3 ± 0.8 | 90.7 ± 0.8 | 13.1 ± 1.3 | 55.6 ± 2 | 23.8 ± 1.7 | 7.5 ± 0.7 |
| EF | 13.9 ± 1* | 86.1 ± 1* | 12.3 ± 1.4 | 57.3 ± 2.1 | 19.2 ± 1.8 | 11.2 ± 1.3* | |
| D3 | DF | 16.2 ± 0.9 | 83.8 ± 0.9 | 15 ± 1.7 | 46.9 ± 2 | 26.1 ± 1.7 | 12 ± 1.3 |
| EF | 12.9 ± 0.4* | 87.1 ± 0.4* | 6.5 ± 1.9* | 42.6 ± 2.7 | 33.2 ± 1.9* | 17.7 ± 2.9* | |
| D7 | DF | 10.1 ± 0.4 | 89.9 ± 0.4 | 4.7 ± 0.8 | 48.3 ± 2.2 | 42.1 ± 1.9 | 4.9 ± 0.9 |
| EF | 8.1 ± 0.5* | 91.9 ± 0.5* | 4.4 ± 0.4 | 49.9 ± 1.5 | 40.4 ± 1.7 | 5.5 ± 0.7 | |
| D10 | DF | 11 ± 0.5 | 89 ± 0.5 | 2.5 ± 0.5 | 52.1 ± 1.5 | 42.4 ± 1.3 | 3 ± 0.6 |
| EF | 8.2 ± 0.6* | 91.8 ± 0.6* | 2.8 ± 0.4 | 49.9 ± 1.4 | 43.5 ± 1.4 | 3.8 ± 0.6 | |
Ratios of cell sub-types, relative to the total number villus/crypt cells. Values are means ± SEM, asterisks, and bold lettering mark significant differences between DF chicks and EF chicks at each age by t-test, p < 0.05.