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. 2020 Dec 17;11:576516. doi: 10.3389/fimmu.2020.576516

Figure 1.

Figure 1

Dietary CLA blend decreased aortic miR-155 expression in ApoE−/− mice. ApoE−/− mice were fed a ND or a 1% CD for 12 weeks or a 1% CD for 12 weeks with the final 4 weeks supplemented with 1% CLA blend (1% CD + CLA). Aortae were dissected and RNA was isolated. Previously, transcriptomic analysis was performed on murine aortae from the 1% CD + CLA group and the 1% CD group by McCarthy et al. (A) In silico, IPA analysis was performed to identify upstream regulators of the CLA-responsive gene set (n = 1082; FDR p < 0.05; Fold-change >1.7). The activation Z-score (≥ 2 or ≤ −2) and upstream miRNA regulators identified were graphed, the overlap p-value was set to p < 0.05. (B) miR-155 expression was analyzed by qRT-PCR using RNU6B as the reference gene. (C) BCL-6 and (D) SHIP-1 expression analyzed by qRT-PCR using 18S as the reference gene and graphed as fold over control (F.O.C.). qRT-PCR results are expressed as mean ± SEM (4 ≤ n ≤ 9). Statistical analysis was performed using a Mann-Whitney U test. *p < 0.05 was considered statistically significant. * over the columns are comparisons with the ND, control group. Significance between other groups is indicated by capped lines.