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. 2020 Dec 17;11:587176. doi: 10.3389/fphar.2020.587176

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Copyright © 2020 Mao, Li, Feng, Liu, Tian, Bian, Li, Hu, Zhang, Ji and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bufei Jianpi formula (BJF) regulated the adenosine monophosphate-activated protein kinase (AMPK) signaling pathway in cigarette smoke extract (CSE)-exposed L6 cells. (A–C) Real-time polymerase chain reaction validation of mammalian target of rapamycin (mTOR), PPARγ, and SIRT1 mRNA expression levels and (D–G) Representative Western blot analysis of mTOR, peroxisome proliferator-activated receptor (PPAR)-γ, and sirtuin 1 (SIRT1) protein expression levels. Data are presented as mean ± SD. ∗∗p < 0.01, ∗p < 0.05 vs. control group; ^## p < 0.01, ^# p < 0.05 vs. model group; ^ΔΔ P < 0.01, ^Δ P < 0.05 vs. BJF group; ^▲▲ p < 0.01, ^▲ p < 0.05 vs. AICAR group. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.