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. 2020 Dec 17;11:554795. doi: 10.3389/fmicb.2020.554795

FIGURE 1.

FIGURE 1

Triphasic response of Mtb culture to rifampicin and validation of 5-FAM-rifampicin as a permeability probe for rifampicin. (A) Triphasic response of Mtb culture to 10× MBC rifampicin. (B) The possible structural isomers of 5-FAM-rifampicin conjugates. The carboxyl group of the 5-FAM can form an ester bond with any one of the hydroxyl groups in the aliphatic ring of rifampicin, generating two possible isomers. Ester bond is shown in red circle. This structure of 5-FAM-rifampicin is a modified version of the independent structures of the rifampicin and 5-FAM shown in the Supplementary Figures S3A,B in Jakkala and Ajitkumar (2019). (C) Standard graph showing the 5-FAM-rifampicin permeability into MLP cells at two-fold increasing concentrations. (D) Bar graph showing the growth inhibition of Staphylococcus aureus by rifampicin and 5-FAM-rifampicin using agar diffusion assay.