TABLE 5.
Comparison of genes involved in the synthesis of resistance inducers in strains ZF129 and ZF197 as well as strains HY96-2, SQR-21, SC2, and J (sequence similarity is expressed as a percentage of the amino acid identity).
| Resistance inducer | Gene | Product | ZF129 |
ZF197 |
HY96-2 |
SQR-21 |
SC2 |
J |
|||||
| Locus tag | Locus tag | Identity (%) | Locus tag | Identity (%) | Locus tag | Identity (%) | Locus tag | Identity (%) | Locus tag | Identity (%) | |||
| 2,3-Butanediol | budA | Acetolactate decarboxylase | WP_016821069.1 | WP_149032860.1 | 96.37% | WP_016821069.1 | 100.00% | WP_025364704.1 | 99.60% | WP_013370859.1 | 97.58% | WP_025722973.1 | 95.56 |
| ilvN | Acetolactate synthase small subunit | WP_016820552.1 | WP_016820552.1 | 100.00 | WP_016820552.1 | 100.00 | WP_016820552.1 | 100.00 | WP_013370080.1 | 99.38 | WP_023987760.1 | 98.76 | |
| Methanethiol | metH | Methionine synthase | WP_044788161.1 | WP_149033329.1 | 98.25 | WP_045244486.1 | 99.74 | WP_039269038.1 | 99.74 | WP_043885986.1 | 99.56 | WP_069289676.1 | 97.99 |
| metE | 5-methyltetrahydro-pteroyltriglutamate- homocysteine S-methyltransferase | WP_017428578.1 | WP_149034631.1 | 94.43 | WP_039276423.1 | 99.35 | WP_025366075.1 | 99.09 | WP_013373554.1 | 96.11 | WP_069290615.1 | 93.39 | |
| Isoprene | idi | Type 2 isopentenyl-diphosphate Delta-isomerase | WP_126666423.1 | WP_149034550.1 | 94.81 | WP_103042605.1 | 95.63 | WP_025366018.1 | 98.63 | WP_013373457.1 | 97.81 | WP_069290552.1 | 92.62 |
| lytB | 4-hydroxy-3 -methylbut-2-enyl diphosphate reductase | WP_025364457.1 | WP_064795308.1 | 97.80 | WP_031463165.1 | 99.06 | WP_025364457.1 | 100 | WP_014599573.1 | 98.43 | WP_013309434.1 | 97.80 | |
| gcpE | Flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase | WP_017427410.1 | WP_063212213.1 | 99.73 | WP_017427410.1 | 100.00 | WP_017427410.1 | 100.00 | WP_013372694.1 | 99.73 | WP_025718670.1 | 99.19 | |
| ispF | 2-C-methyl-D-erythritol 2,4-cyclodiphosp-hate synthase | WP_016818638.1 | WP_063211188.1 | 98.10 | WP_016818638.1 | 100.00 | WP_016818638.1 | 100.00 | WP_013373286.1 | 98.10 | WP_025723806.1 | 96.84 | |
| ispE | 4-(cytidine 5’-diphospho)-2-C-methyl-D-erythritol kinase | WP_025676573.1 | WP_149034923.1 | 99.30 | WP_025676573.1 | 100.00 | WP_016818920.1 | 99.65 | WP_013368672.1 | 99.65 | WP_069290753.1 | 98.94 | |